Generator

Part:BBa_I766998

Designed by: Jimmy Jie Min Huang   Group: iGEM07_UCSF   (2007-08-16)

YopJ-RR under inducible promotor

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the Fig1 promotor, inducible by activation of the mating pathway. The effector protein is fused to an RR leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2251
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2251
    Illegal NotI site found at 2810
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1300
    Illegal BamHI site found at 1141
    Illegal BamHI site found at 2793
    Illegal XhoI site found at 1891
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2251
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2251
    Illegal NgoMIV site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1069
    Illegal SapI.rc site found at 1093


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Parameters
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